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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Mouse CD45 (30-F11)

CD45 Monoclonal Antibody for IF, FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

IF, FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

30-F11

Cat no : CL647-65087

Synonyms

B220, Cd45, CD45R, loc, Ly 5, Lyt 4, Ptprc, T200

Validation Data Gallery

Applications:
  • IF Staining of mouse splenocytes using CL647-65087

    IF Staining of mouse splenocytes using CL647-65087

    Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD45 (CL647-65087, Clone: 30-F11) at dilution of 1:400.

  • FC experiment of mouse splenocytes using CL647-65087

    FC experiment of mouse splenocytes using CL647-65087

    1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD45 (CL647-65087, Clone: 30-F11) (red) or isotype control antibody (blue). Cells were not fixed.

Tested Applications

Positive IF detected inmouse splenocytes
Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65087-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65087 targets CD45 in IF, FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Mouse thymus or spleen 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
GenBank accession numberBC028512
Gene symbol Ptprc
Gene ID (NCBI) 19264
RRIDAB_2883673
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 CD45 antibody CL647-65087Download protocol
Standard Protocols
Click here to view our Standard Protocols