CoraLite® Plus 488 Anti-Mouse CD45 (30-F11)
CD45 Monoclonal Antibody for FC, IF
Host / Isotype
Rat / IgG2b
Reactivity
Mouse
Applications
IF, FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
30-F11
Cat no : CL488-65087
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | mouse splenocytes |
Positive FC detected in | mouse splenocytes |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:200-1:800 |
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
Sample-dependent, check data in validation data gallery |
Product Information
CL488-65087 targets CD45 in IF, FC applications and shows reactivity with Mouse samples.
Tested Reactivity | Mouse |
Host / Isotype | Rat / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | Mouse thymus or spleen 相同性解析による交差性が予測される生物種 |
Full Name | protein tyrosine phosphatase, receptor type, C |
GenBank accession number | BC028512 |
Gene symbol | Ptprc |
Gene ID (NCBI) | 19264 |
RRID | AB_2883169 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. |
Background Information
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 CD45 antibody CL488-65087 | Download protocol |
FC protocol for CL Plus 488 CD45 antibody CL488-65087 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |