CoraLite® Plus 647 Anti-Mouse CD44 (IM7)

CD44 Monoclonal Antibody for FC, IF

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Human, Mouse

Applications

IF, FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

IM7

Cat no : CL647-65117

Synonyms

AU023126, AW121933, AW146109, Cd44, CD44 antigen, HERMES, Ly 24, Pgp 1



Tested Applications

Positive IF detected inmouse splenocytes
Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65117-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65117 targets CD44 in IF, FC applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Dexamethasone-induced myeloid leukemia M1 cells 相同性解析による交差性が予測される生物種
Full Name CD44 antigen
GenBank accession numberBC051388
Gene symbol Cd44
Gene ID (NCBI) 12505
RRIDAB_2883693
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 CD44 antibody CL647-65117Download protocol
Standard Protocols
Click here to view our Standard Protocols