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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PE Anti-Mouse CD40 (1C10)

CD40 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

1C10

Cat no : PE-65062

Synonyms

AI326936, Bp50, Cd40, CD40 antigen, GP39, HIGM1, IGM, IMD3, p50, T BAM, Tnfrsf5

Validation Data Gallery

Applications:
  • FC experiment of mouse splenocytes using PE-65062

    FC experiment of mouse splenocytes using PE-65062

    1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.20 ug PE-Anti-Mouse CD40 (PE-65062, clone 1C10) (red) or 0.20 ug PE-rat IgG2a isotype control (blue). Cells were not fixed.

Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

PE-65062 targets CD40 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen sCD40 相同性解析による交差性が予測される生物種
Full Name CD40 antigen
GenBank accession numberBC029254
Gene symbol Cd40
Gene ID (NCBI) 21939
RRIDAB_2883859
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and a stabilizer.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).

Protocols

Product Specific Protocols
FC protocol for PE CD40 antibody PE-65062Download protocol
Standard Protocols
Click here to view our Standard Protocols