Validation Data Gallery
Tested Applications
| Positive FC detected in | BALB/c mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| FC | See 4 publications below |
Product Information
FITC-65062 targets CD40 in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
sCD40 相同性解析による交差性が予測される生物種 |
| Full Name | CD40 antigen |
| GenBank accession number | BC029254 |
| Gene Symbol | Cd40 |
| Gene ID (NCBI) | 21939 |
| RRID | AB_2883752 |
| Conjugate | FITC Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 494 nm / 520 nm |
| Form | |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P27512 |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC CD40 antibody FITC-65062 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol Mesenchymal stem cell-derived extracellular vesicles carrying miR-99b-3p restrain microglial activation and neuropathic pain by stimulating autophagy | ||
Adv Healthc Mater Pathogen-mimicking Nanoparticles Based on Rigid Nanomaterials as an Efficient Subunit Vaccine Delivery System for Intranasal Iimmunization | ||
Int J Biol Macromol Mucoadhesive chitosan-catechol as an efficient vaccine delivery system for intranasal immunization | ||
Int Immunopharmacol Huc-MSCs-derived exosomes attenuate neuropathic pain by inhibiting activation of the TLR2/MyD88/NF-κB signaling pathway in the spinal microglia by targeting Rsad2 |
