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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488 Anti-Human CD40 (G28.5)

CD40 Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

G28.5

Cat no : CL488-65103

Synonyms

B cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDW40, p50, TNFRSF5

Validation Data Gallery

Applications:
  • FC experiment of human peripheral blood lymphocytes using CL488-65103

    FC experiment of human peripheral blood lymphocytes using CL488-65103

    1X10^6 human peripheral blood lymphocytes were surface stained with 5.00 ul/test CoraLite® Plus 488-conjugated Anti-Human CD40 (CL488-65103, Clone: G28.5) (red) or isotype control antibody (blue). Cells were not fixed.

  • FC experiment of Raji using CL488-65103

    FC experiment of Raji using CL488-65103

    1X10^6 Raji cells were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD40 (CL488-65103, Clone:G28.5) (red), or 5 ul Control Antibody. Cells were not fixed .

Tested Applications

Positive FC detected inhuman peripheral blood lymphocytes, Raji cells
For other applications, we recommend the unconjugated version of this antibody, 65103-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL488-65103 targets CD40 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / Mouse IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name CD40 molecule, TNF receptor superfamily member 5
Calculated molecular weight 277 aa, 31 kDa
GenBank accession numberBC012419
Gene symbol CD40
Gene ID (NCBI) 958
RRIDAB_2883177
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD40 antibody CL488-65103Download protocol
Standard Protocols
Click here to view our Standard Protocols