CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5)
CD4 Monoclonal Antibody for FC, IF
Host / Isotype
Rat / IgG2b, kappa
Reactivity
Mouse
Applications
IF, FC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
GK1.5
Cat no : CL647-65104
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive IF detected in | mouse splenocytes |
| Positive FC detected in | mouse splenocytes |
For other applications, we recommend the unconjugated version of this antibody, 65104-1-Ig
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:250-1:1000 |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| IF | See 1 publications below |
Product Information
CL647-65104 targets CD4 in IF, FC applications and shows reactivity with Mouse samples.
| Tested Reactivity | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Mouse CTL clone V4 相同性解析による交差性が予測される生物種 |
| Full Name | CD4 antigen |
| GenBank accession number | BC039137 |
| Gene symbol | Cd4 |
| Gene ID (NCBI) | 12504 |
| RRID | AB_2883682 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.09% sodium azide. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 CD4 antibody CL647-65104 | Download protocol |
| FC protocol for CL Plus 647 CD4 antibody CL647-65104 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Chem Activated platelet membrane nanovesicles recruit neutrophils to exert the antitumor efficiency |
