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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488 Anti-Human CD38 (HIT2)

CD38 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

HIT2

Cat no : CL488-65111

Synonyms

ADP ribosyl cyclase 1, cADPr hydrolase 1, CD38, CD38 molecule, Cyclic ADP ribose hydrolase 1, T10

Validation Data Gallery

Applications:
  • FC experiment of human PBMCs using CL488-65111

    FC experiment of human PBMCs using CL488-65111

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD38 (CL488-65111, Clone:HIT2), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL488-65111

    FC experiment of human PBMCs using CL488-65111

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD38 (CL488-65111, Clone:HIT2), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

Tested Applications

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD38 Ready-To-Use IHC Kit. CD38 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65111-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL488-65111 targets CD38 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name CD38 molecule
Calculated molecular weight 300 aa, 34 kDa
GenBank accession numberBC007964
Gene symbol CD38
Gene ID (NCBI) 952
RRIDAB_2883184
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD38 antibody CL488-65111Download protocol
Standard Protocols
Click here to view our Standard Protocols