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  • KD/KO Validated

CD38 Monoclonal antibody

CD38 Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3C6G4

Cat no : 60006-1-Ig

Synonyms

ADP ribosyl cyclase 1, cADPr hydrolase 1, CD38, CD38 molecule, Cyclic ADP ribose hydrolase 1, T10



Tested Applications

Positive WB detected inJurkat cells, Raji cells
Positive IF detected inNIH/3T3 cells
Positive FC detected inDaudi cells
Planning an IHC experiment? We recommend our IHCeasy CD38 Ready-To-Use IHC Kit. CD38 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

60006-1-Ig targets CD38 in WB, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD38 fusion protein Ag0487 相同性解析による交差性が予測される生物種
Full Name CD38 molecule
Calculated molecular weight 300 aa, 34 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC007964
Gene symbol CD38
Gene ID (NCBI) 952
RRIDAB_716994
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).

Protocols

Product Specific Protocols
WB protocol for CD38 antibody 60006-1-IgDownload protocol
IF protocol for CD38 antibody 60006-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Immunol

PD-1 blockade in subprimed CD8 cells induces dysfunctional PD-1+CD38hi cells and anti-PD-1 resistance.

Authors - Vivek Verma
  • KD Validated
mouseWB

Glia

Activation of astrocytic sigma-1 receptor exerts antidepressant-like effect via facilitating CD38-driven mitochondria transfer.

Authors - Yun Wang
  • KD Validated
human

Cancer Cell Int

Targeting Lymphotoxin Beta and Paired Box 5: a potential therapeutic strategy for soft tissue sarcoma metastasis.

Authors - Runzhi Huang
IF

Cell Rep

Longitudinal analyses reveal distinct immune response landscapes in lung and intestinal tissues from SARS-CoV-2-infected rhesus macaques.

Authors - Huiwen Zheng
human

J Immunol

Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues

Authors - Teresa W-M Fan
mouseWB

J Nutr Biochem

Both prolonged high-fat diet consumption and calorie restriction boost hepatic NAD+ metabolism in mice

Authors - Xiaojing Wei

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Komuraiah (Verified Customer) (07-16-2021)

CD38 expression that I tested in kidney tissue from control and cisplatin induced kidney injury model. This antibody is working great with high specificity with suggested molecular size.

  • Applications: Western Blot
CD38 Antibody Western Blot validation ( dilution) in (Cat no:60006-1-Ig)