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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD35 (E11)

CD35 Monoclonal Antibody for FC, ELISA

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC, ELISA

Conjugate

Unconjugated

CloneNo.

E11

Cat no : 65254-1-Ig

Synonyms

C3b/C4b receptor, C3BR, CD35, Complement receptor type 1, CR1, KN

Validation Data Gallery

Applications:
  • FC experiment of human PBMCs using 65254-1-Ig

    FC experiment of human PBMCs using 65254-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD35 (65254-1-Ig, Clone:E11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or CoraLite®488-Conjugated Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65254-1-Ig

    FC experiment of human PBMCs using 65254-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD35 (65254-1-Ig, Clone:E11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or CoraLite®488-Conjugated Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

Tested Applications

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CR1/CD35 Ready-To-Use IHC Kit. CR1/CD35 primary antibody included.

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65254-1-Ig targets CD35 in FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human CD35 from Acute monocytic leukemia cells and normal blood monocytes 相同性解析による交差性が予測される生物種
Full Name complement component (3b/4b) receptor 1 (Knops blood group)
Calculated molecular weight 224 kDa
GenBank accession numberNM_000573
Gene symbol CR1
Gene ID (NCBI) 1378
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes.

Protocols

Product Specific Protocols
FC protocol for CD35 antibody 65254-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols