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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD33 Monoclonal antibody

CD33 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1B2E5

Cat no : 67135-1-Ig

Synonyms

CD33, CD33 molecule, FLJ00391, gp67, p67, SIGLEC 3, SIGLEC3

Validation Data Gallery

Applications:
  • WB analysis of THP-1 using 67135-1-Ig

    WB analysis of THP-1 using 67135-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67135-1-Ig (CD33 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HL-60 using 67135-1-Ig

    WB analysis of HL-60 using 67135-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 67135-1-Ig (CD33 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 67135-1-Ig

    IHC staining of human spleen using 67135-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67135-1-Ig (CD33 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human spleen using 67135-1-Ig

    IHC staining of human spleen using 67135-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67135-1-Ig (CD33 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of THP-1 using 67135-1-Ig

    IF Staining of THP-1 using 67135-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD33 antibody (67135-1-Ig, Clone: 1B2E5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inTHP-1 cells, HL-60 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67135-1-Ig targets CD33 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD33 fusion protein Ag11402 相同性解析による交差性が予測される生物種
Full Name CD33 molecule
Calculated molecular weight 364 aa, 40 kDa
Observed molecular weight 67-70 kDa
GenBank accession numberBC028152
Gene symbol CD33
Gene ID (NCBI) 945
RRIDAB_2882434
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.

Protocols

Product Specific Protocols
WB protocol for CD33 antibody 67135-1-IgDownload protocol
IHC protocol for CD33 antibody 67135-1-IgDownload protocol
IF protocol for CD33 antibody 67135-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols