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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Mouse CD31 (390)

CD31 Monoclonal Antibody for IF,FC

Host / Isotype

Rat / IgG2a

Reactivity

Mouse

Applications

IF,FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

390

Cat no : CL647-65058

Synonyms

C85791, Cd31, Pecam, PECAM 1, Pecam1

Validation Data Gallery

Applications:
  • IF Staining of bEnd.3 using CL647-65058

    IF Staining of bEnd.3 using CL647-65058

    Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CoraLite® Plus 647 CD31 antibody (CL647-65058, Clone: 390) at dilution of 1:100.

  • FC experiment of mouse splenocytes using CL647-65058

    FC experiment of mouse splenocytes using CL647-65058

    1X10^6 C57BL/6 mouse splenocytes were surface stained with 5.00 ul/test CoraLite® Plus 647-conjugated Anti-Mouse CD31 (CL647-65058, Clone: 390) (red) or isotype control antibody (blue). Cells were not fixed.

Tested Applications

Positive IF detected inbEnd.3 cells
Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65058-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65058 targets CD31 in IF,FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2a
Class Monoclonal
Type Antibody
Immunogen C3H/HeJ mouse hematopoietic progenitor cell line 32D 相同性解析による交差性が予測される生物種
Full Name platelet/endothelial cell adhesion molecule 1
GenBank accession numberBC008519
Gene symbol Pecam1
Gene ID (NCBI) 18613
RRIDAB_2883659
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 CD31 antibody CL647-65058Download protocol
FC protocol for CL Plus 647 CD31 antibody CL647-65058Download protocol
Standard Protocols
Click here to view our Standard Protocols