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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594 Anti-Mouse CD3ε (500-A2)

CD3ε Monoclonal Antibody for FC

Host / Isotype

Hamster / IgG

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

500-A2

Cat no : CL594-65061

Synonyms

AI504783, CD3, Cd3e, CD3epsilon, CD3ε, T3e

Validation Data Gallery

Applications:
  • FC experiment of mouse splenocytes using CL594-65061

    FC experiment of mouse splenocytes using CL594-65061

    1X10^6 mouse splenocytes were surface stained with 0.5 ug CoraLite®594 Anti-Mouse CD3ε (CL594-65061, Clone:500-A2) (red), or 0.5 ug Isotype Control. Cells were not fixed.

Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

CL594-65061 targets CD3ε in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Hamster / IgG
Class Monoclonal
Type Antibody
Immunogen Immune complexes isolated from C6VL-BS thymoma cells 相同性解析による交差性が予測される生物種
Full Name CD3 antigen, epsilon polypeptide
GenBank accession numberBC098236
Gene symbol Cd3e
Gene ID (NCBI) 12501
RRIDAB_2919930
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocols

Product Specific Protocols
FC protocol for CL594 CD3ε antibody CL594-65061Download protocol
Standard Protocols
Click here to view our Standard Protocols