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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using APC Anti-Human CD3 (Hit3a) (APC-65112)

    FC experiment of human peripheral blood lymphocytes using APC-65112

    1X10^6 human peripheral blood lymphocytes were surface stained with 5 ul APC-anti-human CD3 (APC-65112, clone Hit3a) (red) or APC-mouse IgG2a isotype control (blue). Cells were not fixed.

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APC Anti-Human CD3 (Hit3a)

CD3 Monoclonal Antibody for FC
Cat No. APC-65112
Clone No.Hit3a

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

human

Applications

FC

Synonyms

CD3E, CD3 epsilon, CD3-epsilon, Hit3a, T3E

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  APC
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inHuman peripheral blood lymphocytes

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 5 ul per 10^6 cells in 100 μl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-65112 targets CD3 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Calculated molecular weight 207 aa, 23 kDa
GenBank accession numberBC049847
Gene Symbol CD3
Gene ID (NCBI) 916
ENSEMBL Gene IDENSG00000198851
RRIDAB_2883001
Conjugate APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 660 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP07766
Storage Buffer PBS with 0.09% sodium azide and 0.1% gelatin{{ptg:BufferTemp}}7.2
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocols

Product Specific Protocols
FC protocol for APC CD3 antibody APC-65112Download protocol
Standard Protocols
Click here to view our Standard Protocols