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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human PD-1/CD279 (J110)

PD-1/CD279 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

J110

Cat no : 65119-1-Ig

Synonyms

CD279, hPD 1, hPD l, PD1, PD-1, PDCD1, programmed cell death 1, Protein PD 1, SLEB2

Validation Data Gallery

Applications:
  • FC experiment of human PBMCs using 65119-1-Ig

    FC experiment of human PBMCs using 65119-1-Ig

    1x10^6 PHA treated (3 day) human PBMCs were surface stained with 0.2 ug Anti-Human PD-1/CD279 (65119-1-Ig, Clone:J110) (right) or Mouse IgG1 Isotype Control (left). 1x10^6 untreated human PBMCs were surface stained with 0.2 ug Anti-Human PD-1/CD279 (65119-1-Ig, Clone:J110) (center). All cells were then stained with CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8) followed by staining with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65119-1-Ig

    FC experiment of human PBMCs using 65119-1-Ig

    1x10^6 PHA treated (3 day) human PBMCs were surface stained with 0.2 ug Anti-Human PD-1/CD279 (65119-1-Ig, Clone:J110) (red) or Mouse IgG1 Isotype Control (blue). 1x10^6 untreated human PBMCs were surface stained with 0.2 ug Anti-Human PD-1/CD279 (65119-1-Ig, Clone:J110) (black dashed). All cells were then stained with CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed. Lymphocytes were gated.

Tested Applications

Positive FC detected inPHA treated human PBMCs
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65119-1-Ig targets PD-1/CD279 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name programmed cell death 1
Calculated molecular weight 288 aa, 32 kDa
GenBank accession numberBC074740
Gene symbol PDCD1
Gene ID (NCBI) 5133
RRIDAB_2918414
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).

Protocols

Product Specific Protocols
FC protocol for PD-1/CD279 antibody 65119-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols