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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488 Anti-Mouse CD25 (PC61.5)

CD25 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, lambda

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

PC61.5

Cat no : CL488-65137

Synonyms

CD25, Il2r, Il2ra, Il-2ra, Ly 43

Validation Data Gallery

Applications:
  • FC experiment of mouse splenocytes using CL488-65137

    FC experiment of mouse splenocytes using CL488-65137

    1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD4 and 0.125 ug CoraLite® Plus 488 Anti-Mouse CD25 (CL488-65137, Clone: PC61.5) or 0.125 ug CoraLite® Plus 488-conjugated Rat IgG1 isotype control. Cells were not fixed.

Tested Applications

Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65137-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

CL488-65137 targets CD25 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG1, lambda
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name interleukin 2 receptor, alpha chain
GenBank accession numberBC114437
Gene symbol Il2ra
Gene ID (NCBI) 16184
RRIDAB_2883198
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells (PMID: 22585674).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD25 antibody CL488-65137Download protocol
Standard Protocols
Click here to view our Standard Protocols