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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488 Anti-Human CD25 (BC96)

CD25 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

BC96

Cat no : CL488-65096

Synonyms

CD25, IDDM10, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha, IL2 RA, IL2R, IL2RA, IL-2RA, interleukin 2 receptor, alpha, p55, TAC antigen, TCGFR

Validation Data Gallery

Applications:
  • FC experiment of human peripheral blood lymphocytes using CL488-65096

    FC experiment of human peripheral blood lymphocytes using CL488-65096

    1X10^6 human peripheral blood mononuclear cells (PBMCs) were surface stained with APC Anti-Human CD4 (APC-65134, Clone: OKT4) and 5.00 ul CoraLite® Plus 488-conjugated Anti-Human CD25 (CL488-65096, Clone: BC96). Cells were not fixed.

  • FC experiment of human peripheral blood lymphocytes using CL488-65096

    FC experiment of human peripheral blood lymphocytes using CL488-65096

    1X10^6 human peripheral blood mononuclear cells (PBMCs) were surface stained with APC Anti-Human CD4 (APC-65134, Clone: OKT4) and CoraLite® Plus 488-conjugated Mouse IgG1 isotype control antibody. Cells were not fixed.

Tested Applications

Positive FC detected inhuman peripheral blood lymphocytes
For other applications, we recommend the unconjugated version of this antibody, 65096-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL488-65096 targets CD25 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name interleukin 2 receptor, alpha
Calculated molecular weight 272 aa, 31 kDa
GenBank accession numberBC156627
Gene symbol IL2RA
Gene ID (NCBI) 3559
RRIDAB_2883175
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD25 antibody CL488-65096Download protocol
Standard Protocols
Click here to view our Standard Protocols