Validation Data Gallery

Filter: AllFC

FC experiment of human PBMCs using 65096-1-Ig
1x10^6 human PBMCs were stimulated with Human CD3/CD28 T cell Activation Beads Kit (KMS310) for 2 days then surface stained with 0.2 ug Anti-Human CD25 (65096-1-Ig, Clone: BC96) (right) or Mouse IgG1 Isotype Control (left). 1x10^6 unstimulated human PBMCs were surface stained with 0.2 ug Anti-Human CD25 (65096-1-Ig, Clone:BC96) (center). All samples were then stained with CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) at dilution 1:1000 followed by CL488-conjugated Anti-Human CD4. Cells were not fixed. Lymphocytes were gated.
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1x10^6 human PBMCs were stimulated with Human CD3/CD28 T cell Activation Beads Kit (KMS310) for 2 days then surface stained with 0.2 ug Anti-Human CD25 (65096-1-Ig, Clone: BC96) (right) or Mouse IgG1 Isotype Control (left). 1x10^6 unstimulated human PBMCs were surface stained with 0.2 ug Anti-Human CD25 (65096-1-Ig, Clone:BC96) (center). All samples were then stained with CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) at dilution 1:1000 followed by CL488-conjugated Anti-Human CD4. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 65096-1-Ig
1x10^6 human PBMCs were stimulated with Human CD3/CD28 T cell Activation Beads Kit (KMS310) for 2 days then surface stained with 0.2 ug Anti-Human CD25 (65096-1-Ig, Clone: BC96) (red) or Mouse IgG1 Isotype Control (blue). 1x10^6 unstimulated human PBMCs were surface stained with 0.2 ug Anti-Human CD25 (65096-1-Ig, Clone:BC96) (black dashed). All samples were then stained with CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.
×
1x10^6 human PBMCs were stimulated with Human CD3/CD28 T cell Activation Beads Kit (KMS310) for 2 days then surface stained with 0.2 ug Anti-Human CD25 (65096-1-Ig, Clone: BC96) (red) or Mouse IgG1 Isotype Control (blue). 1x10^6 unstimulated human PBMCs were surface stained with 0.2 ug Anti-Human CD25 (65096-1-Ig, Clone:BC96) (black dashed). All samples were then stained with CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

Anti-Human CD25/IL-2RA (BC96)

CD25/IL-2RA Monoclonal Antibody for FC

Cat No. 65096-1-Ig

Clone No.BC96

Publications(2)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

Synonyms

CD25, BC96, IL2 RA, IL 2R subunit alpha, IL 2 receptor subunit alpha

Formulation: PBS, Azide

PBS, Azide

Conjugate: Unconjugated

Unconjugated

Atlantic Blue™ Cardinal Red™ CoraLite® Plus 405 CoraLite® Plus 488 CoraLite® Plus 555 CoraLite® Plus 594 CoraLite® Plus 647 CoraLite® Plus 750 FITC Plus PE

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive FC detected in

human PBMCs

Recommended dilution

Application	Dilution
Flow Cytometry (FC)	FC : 0.2 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

FC	See 1 publications below

Product Information

65096-1-Ig targets CD25/IL-2RA in FC applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Immunogen	N/A 相同性解析による交差性が予測される生物種
Full Name	interleukin 2 receptor, alpha
Calculated molecular weight	272 aa, 31 kDa
GenBank accession number	BC156627
Gene Symbol	IL2RA
Gene ID (NCBI)	3559
RRID	AB_2918398
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Affinity purification
UNIPROT ID	P01589
Storage Buffer	PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3
Storage Conditions	Store at 2-8°C. Stable for one year after shipment.

Background Information

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).

Protocols

Product Specific Protocols
FC protocol for CD25/IL-2RA antibody 65096-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	FC	DNA Cell Biol Upregulated Expression of microRNA-16 Correlates with Th17/Treg Cell Imbalance in Patients with Rheumatoid Arthritis. Authors - Yuan-Hao Wu View Article
		Allergy Exploring CD26-/lo subpopulations of lymphocytes in asthma phenotype and severity: A novel CD4+ T cell subset expressing archetypical granulocyte proteins Authors - Sara Vázquez-Mera View Article

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