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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated CD23 Monoclonal antibody

CD23 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4C7C9

Cat no : CL594-60208

Synonyms

BLAST 2, CD23, CD23A, CLEC4J, Fc epsilon RII, FCE2, FCER2, IGEBF, Lymphocyte IgE receptor

Validation Data Gallery

Applications:
  • IF Staining of human tonsillitis using CL594-60208

    IF Staining of human tonsillitis using CL594-60208

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL594-60208 (CD23 antibody) at dilution of 1:50 .

  • IF Staining of human tonsillitis using CL594-60208

    IF Staining of human tonsillitis using CL594-60208

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL594-60208 (CD23 antibody) at dilution of 1:50 .

  • IF Staining of human lymphoma using CL594-60208

    IF Staining of human lymphoma using CL594-60208

    Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using CoraLite®594 CD23 antibody (CL594-60208, Clone: 4C7C9 ) at dilution of 1:200.

  • IF Staining of human lymphoma using CL594-60208

    IF Staining of human lymphoma using CL594-60208

    Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using CoraLite®594 CD23 antibody (CL594-60208, Clone: 4C7C9 ) at dilution of 1:200.

  • FC experiment of U-937 using CL594-60208

    FC experiment of U-937 using CL594-60208

    1X10^6 U-937 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CD23 (CL594-60208, Clone:4C7C9) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inhuman tonsillitis tissue, human lymphoma tissue
Planning an IHC experiment? We recommend our IHCeasy CD23 Ready-To-Use IHC Kit. CD23 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60208-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-60208 targets CD23 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD23 fusion protein Ag0425 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgE, low affinity II, receptor for (CD23)
Calculated molecular weight 321 aa, 36 kDa
GenBank accession numberBC064417
Gene symbol FCER2
Gene ID (NCBI) 2208
RRIDAB_2883442
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells. And the antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Protocols

Product Specific Protocols
IF protocol for CL594 CD23 antibody CL594-60208Download protocol
Standard Protocols
Click here to view our Standard Protocols