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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488 Anti-Mouse CD223 (C9B7W)

CD223 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, kappa

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

C9B7W

Cat no : CL488-65098

Synonyms

CD223, LAG 3, Lag3, Ly66, lymphocyte activation gene 3

Validation Data Gallery

Applications:
  • FC experiment of BALB/c mouse splenocytes using CL488-65098

    FC experiment of BALB/c mouse splenocytes using CL488-65098

    1X10^6 Con A-stimulated (3 ug/ml Con A, day-3) BALB/c mouse splenocytes were surface stained with CoraLite® Plus 647-Conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and 5 ul CoraLite® Plus 488 Anti-Mouse CD223 (CL488-65098, Clone: C9B7W). Cells were not fixed.

  • FC experiment of BALB/c mouse splenocytes using CL488-65098

    FC experiment of BALB/c mouse splenocytes using CL488-65098

    1X10^6 Con A-stimulated (3 ug/ml Con A, day-3) BALB/c mouse splenocytes were surface stained with CoraLite® Plus 647-Conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and CoraLite® Plus 488-Rat IgG1 isotype control. Cells were not fixed.

Tested Applications

Positive FC detected inBALB/c mouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65098-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL488-65098 targets CD223 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Murine CD223-Ig fusion protein 相同性解析による交差性が予測される生物種
Full Name lymphocyte-activation gene 3
GenBank accession numberBC120591
Gene symbol Lag3
Gene ID (NCBI) 16768
RRIDAB_2919262
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Lymphocyte activation gene 3 protein (LAG-3), also known as CD223, is a type I transmembrane protein belonging to the immunoglobulin superfamily (PMID: 1692078). It is a CD4-related molecule that binds MHC class II with high affinity (PMID: 1692078; 9159144). LAG-3 is expressed on activated T and NK cells. Besides, natural CD4(+)CD25(+) Tregs express LAG-3 upon activation (PMID: 15485628). LAG-3 negatively regulates T-cell function and homeostasis, and contributes to the suppressor activity of Tregs (PMID: 14644131; 15485628).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD223 antibody CL488-65098Download protocol
Standard Protocols
Click here to view our Standard Protocols