• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488 Anti-Mouse CD134 (OX-86)

CD134 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, kappa

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

OX-86

Cat no : CL488-65136

Synonyms

ACT35, CD134, Ly 70, Ox40, Tnfrsf4, Txgp1, TXGP1L

Validation Data Gallery

Applications:
  • FC experiment of BALB/c mouse splenocytes using CL488-65136

    FC experiment of BALB/c mouse splenocytes using CL488-65136

    1X10^6 Con A-stimulated (3 ug/ml, 2 days) BALB/c mouse splenocytes were surface stained with CoraLite® Plus 647-Conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and 5 ul CoraLite® Plus 488 Anti-Mouse CD134 (CL488-65136, Clone: OX-86). Cells were not fixed.

  • FC experiment of BALB/c mouse splenocytes using CL488-65136

    FC experiment of BALB/c mouse splenocytes using CL488-65136

    1X10^6 Con A-stimulated (3 ug/ml, 2 days) BALB/c mouse splenocytes were surface stained with CoraLite® Plus 647-Conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and CoraLite® Plus 488-Rat IgG1 isotype control. Cells were not fixed.

Tested Applications

Positive FC detected inCon A-stimulated mouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65136-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL488-65136 targets CD134 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 4
GenBank accession numberBC065782
Gene symbol Tnfrsf4
Gene ID (NCBI) 22163
RRIDAB_2919267
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD134, also known as OX40 and TNFRSF4, is a member of the TNFR-superfamily of receptors (PMID: 2828930; 9766631). It is a type I transmembrane protein predominantly expressed on activated T cells which include CD4 and CD8 T cells, Th2, Th1, and Th17 cells, as well as regulatory T cells (Tregs) (PMID: 20307208). CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule (PMID: 26215166). CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmune diseases, cancer and infectious disease (PMID: 26215166; 19426222).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD134 antibody CL488-65136Download protocol
Standard Protocols
Click here to view our Standard Protocols