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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APC Anti-Mouse CD134 (OX-86)

CD134 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, kappa

Reactivity

Mouse

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

OX-86

Cat no : APC-65136

Synonyms

ACT35, CD134, Ly 70, Ox40, Tnfrsf4, Txgp1, TXGP1L

Validation Data Gallery

Applications:
  • FC experiment of BALB/C mouse splenocytes using APC-65136

    FC experiment of BALB/C mouse splenocytes using APC-65136

    1X10^6 unstimulated or anti-CD3/CD28 stimulated (2 days) mouse splenocytes were surface co-stained with FITC Plus Anti-Mouse CD3 (17A2) (FITC-65077, Clone: 17A2) and 0.2 ug APC Anti-Mouse CD134 (APC-65136, Clone: OX-86). Cells were not fixed.

Tested Applications

Positive FC detected inAnti-CD3/CD28 treated mouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

APC-65136 targets CD134 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Recombinant mouse OX-40-CD4 chimeric protein 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 4
GenBank accession numberBC065782
Gene symbol Tnfrsf4
Gene ID (NCBI) 22163
RRIDAB_2883017
Conjugate APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 660 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD134, also known as OX40 and TNFRSF4, is a member of the TNFR-superfamily of receptors (PMID: 2828930; 9766631). It is a type I transmembrane protein predominantly expressed on activated T cells which include CD4 and CD8 T cells, Th2, Th1, and Th17 cells, as well as regulatory T cells (Tregs) (PMID: 20307208). CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule (PMID: 26215166). CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmune diseases, cancer and infectious disease (PMID: 26215166; 19426222).

Protocols

Product Specific Protocols
FC protocol for APC CD134 antibody APC-65136Download protocol
Standard Protocols
Click here to view our Standard Protocols