CoraLite® Plus 488 Anti-Mouse CD11c (N418)
CD11c Monoclonal Antibody for FC, IF
Host / Isotype
Armenian Hamster / IgG
Reactivity
Mouse
Applications
IF, FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
N418
Cat no : CL488-65130
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | mouse splenocytes |
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-65130 targets CD11c in IF, FC applications and shows reactivity with Mouse samples.
| Tested Reactivity | Mouse |
| Host / Isotype | Armenian Hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Mouse spleen dendritic cells 相同性解析による交差性が予測される生物種 |
| Full Name | integrin alpha X |
| GenBank accession number | BC167225 |
| Gene symbol | Itgax |
| Gene ID (NCBI) | 16411 |
| RRID | AB_2883193 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CD11c antibody CL488-65130 | Download protocol |
| FC protocol for CL Plus 488 CD11c antibody CL488-65130 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
