Validation Data Gallery
Tested Applications
| Positive FC detected in | mouse bone marrow cells, human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 3 publications below |
| FC | See 17 publications below |
Product Information
FITC-65055 targets CD11b in IF, FC applications and shows reactivity with Human,Mouse,Cynomolgus monkey,Guinea pig,Rabbit samples.
| Tested Reactivity | Human,Mouse,Cynomolgus monkey,Guinea pig,Rabbit |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes 相同性解析による交差性が予測される生物種 |
| Full Name | integrin alpha M |
| Calculated molecular weight | 127 kDa |
| GenBank accession number | BC156991 |
| Gene Symbol | CD11b |
| Gene ID (NCBI) | 16409 |
| RRID | AB_2883745 |
| Conjugate | FITC Plus Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 495 nm / 524 nm |
| 激发激光 | Blue laser (488 nm) |
| Form | |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | E9Q604 |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC Plus CD11b antibody FITC-65055 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bioact Mater Targeted transplantation of engineered mitochondrial compound promotes functional recovery after spinal cord injury by enhancing macrophage phagocytosis | ||
Nat Commun Targeting phagocytosis for amyloid-β clearance: implications of morphology remodeling and microglia activation probed by bifunctional chimaeras | ||
ACS Nano Bioengineered Nanospores Selectively Blocking LC3-Associated Phagocytosis in Tumor-Associated Macrophages Potentiate Antitumor Immunity | ||
Cell Death Dis Lactate secreted by PKM2 upregulation promotes Galectin-9-mediated immunosuppression via inhibiting NF-κB pathway in HNSCC. | ||
Cancer Lett BMP9 potentiates immunotherapy in triple-negative breast cancer by suppressing Tregs infiltration via the PRKDC-CCL2 axis. | ||
Cell Commun Signal Transfer of cGAMP from neuron to microglia activates microglial type I interferon responses after subarachnoid hemorrhage |