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Species Reactivity
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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 555 Anti-Rat CD11b (OX42)

CD11b Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Rat

Applications

FC

Conjugate

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

OX42

Cat no : CL555-65229

Synonyms

Cd11b, integrin alpha M, Itgam

Validation Data Gallery

Applications:
  • FC experiment of rat splenocytes using CL555-65229

    FC experiment of rat splenocytes using CL555-65229

    1X10^6 rat splenocytes were surface stained with 0.5 ug CoraLite® Plus 555 Anti-Rat CD11b (CL555-65229, Clone:OX42) or 0.5 ug Isotype Control. Cells were not fixed.

Tested Applications

Positive FC detected inrat splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65229-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

CL555-65229 targets CD11b in FC applications and shows reactivity with Rat samples.

Tested Reactivity Rat
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Rat peritoneal macrophages 相同性解析による交差性が予測される生物種
Full Name integrin alpha M
Calculated molecular weight127 kDa
GenBank accession numberNM_012711
Gene symbol Itgam
Gene ID (NCBI) 25021
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission maxima wavelengths554 nm / 570 nm
Form Liquid
Purification Method N/A
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Protocols

Product Specific Protocols
FC protocol for CL Plus 555 CD11b antibody CL555-65229Download protocol
Standard Protocols
Click here to view our Standard Protocols