CoraLite® Plus 488 Anti-Mouse CD11b (M1/70)
CD11b Monoclonal Antibody for IF, FC
Host / Isotype
Rat / IgG2b, kappa
Reactivity
Mouse, Human and More (2)
Applications
IF, FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
M1/70
Cat no : CL488-65055
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | mouse splenocytes |
| Positive FC detected in | mouse bone marrow cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| IF | See 1 publications below |
| FC | See 1 publications below |
Product Information
CL488-65055 targets CD11b in IF, FC applications and shows reactivity with Mouse, Human samples.
| Tested Reactivity | Mouse, Human |
| Cited Reactivity | rat, mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes 相同性解析による交差性が予測される生物種 |
| Full Name | integrin alpha M |
| GenBank accession number | BC156991 |
| Gene symbol | Itgam |
| Gene ID (NCBI) | 16409 |
| RRID | AB_2883153 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CD11b antibody CL488-65055 | Download protocol |
| FC protocol for CL Plus 488 CD11b antibody CL488-65055 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Vet Res Curcumin-dependent phenotypic transformation of microglia mediates resistance to pseudorabies-induced encephalitis | ||
Biomed Pharmacother Mitochondrion-NLRP3 inflammasome activation in macrophages: A novel mechanism of the anti-inflammatory effect of Notopterygium in rheumatoid arthritis treatment |
