Validation Data Gallery
Tested Applications
| Positive FC detected in | TF-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 20 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 20 µl per 10^6 cells in a 100 µl suspension or 20 µl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
PE-65154 targets CD117/c-Kit in FC applications and shows reactivity with human, bovine samples.
| Tested Reactivity | human, bovine |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
MOLM-1 megakaryocytic cells 相同性解析による交差性が予測される生物種 |
| Full Name | v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog |
| Calculated molecular weight | 976 aa, 110 kDa |
| GenBank accession number | BC071593 |
| Gene Symbol | CD117 |
| Gene ID (NCBI) | 3815 |
| RRID | AB_2883933 |
| Conjugate | PE Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 496 nm, 565 nm / 578 nm |
| Form | |
| Form | Liquid |
| Purification Method | The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| UNIPROT ID | P10721 |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PE CD117/c-Kit antibody PE-65154 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
