Validation Data Gallery
Tested Applications
| Positive IF-P detected in | mouse liver tissue |
| Positive FC detected in | bEnd.3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
65048-1-Ig targets Endoglin/CD105 in IF-P, FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Inflamed mouse skin 相同性解析による交差性が予測される生物種 |
| Full Name | endoglin |
| GenBank accession number | BC029080 |
| Gene Symbol | CD105 |
| Gene ID (NCBI) | 13805 |
| RRID | AB_2918360 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | Q63961 |
| Storage Buffer | Borate buffered saline{{ptg:BufferTemp}}8.2 |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Endoglin/CD105 antibody 65048-1-Ig | Download protocol |
| FC protocol for Endoglin/CD105 antibody 65048-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bone Radial extracorporeal shockwave promotes osteogenesis-angiogenesis coupling of bone marrow stromal cells from senile osteoporosis via activating the Piezo1/CaMKII/CREB axis |