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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488 Anti-Mouse CD103 (2E7)

CD103 Monoclonal Antibody for FC

Host / Isotype

Hamster / IgG

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E7

Cat no : CL488-65047

Synonyms

A530055J10, alpha E1, alpha M290, aM290, CD103, Itgae

Validation Data Gallery

Applications:
  • FC experiment of mouse splenocytes using CL488-65047

    FC experiment of mouse splenocytes using CL488-65047

    1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 0.5 ug CoraLite® Plus 488 Anti-Mouse CD103 (CL488-65047, Clone:2E7) or 0.5 ug Isotype Control. Cells were not fixed.

Tested Applications

Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65047-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

CL488-65047 targets CD103 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Hamster / IgG
Class Monoclonal
Type Antibody
Immunogen C57BL/6J mouse intestinal intraepithelial lymphocytes (IEL) 相同性解析による交差性が予測される生物種
Full Name integrin alpha E, epithelial-associated
GenBank accession numberBC150690
Gene symbol Itgae
Gene ID (NCBI) 16407
RRIDAB_2883147
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD103, also known as integrin alpha-E (ITGAE) or integrin alpha-IEL, is a type I transmembrane integrin protein that binds integrin beta 7 to form Integrin alpha-E beta-7 which is a receptor for E-cadherin and mediates adhesion of T lymphocytes to epithelial cells (PMID: 7969453; 8468482). CD103 is expressed on intraepithelial lymphocyte (IEL) T cells (both alpha/beta T cells and gamma/delta T cells), some peripheral regulatory T cells (Tregs), lamina propria T cells, and a subset of dendritic cells in the gut mucosa and mesenteric lymph nodes (PMID: 12242333; 15608520; 16216890).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD103 antibody CL488-65047Download protocol
Standard Protocols
Click here to view our Standard Protocols