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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

CCT8 Polyclonal antibody

CCT8 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 12263-1-AP

Synonyms

C21orf112, CCT theta, CCT8, Cctq, D21S246, KIAA0002, PRED71, TCP 1 theta

Validation Data Gallery

Applications:
  • WB analysis using 12263-1-AP

    WB analysis using 12263-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12263-1-AP (CCT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse kidney using 12263-1-AP

    WB analysis of mouse kidney using 12263-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12263-1-AP (CCT8 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 12263-1-AP

    WB analysis of HEK-293 using 12263-1-AP

    WB result of CCT8 antibody (12263-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT8 transfected HEK-293 cells.

  • IP experiment of Jurkat using 12263-1-AP

    IP experiment of Jurkat using 12263-1-AP

    IP result of anti-CCT8 (IP:12263-1-AP, 4ug; Detection:12263-1-AP 1:500) with Jurkat cells lysate 3160 ug.

  • IF Staining of SW480 using 12263-1-AP

    IF Staining of SW480 using 12263-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SW480 cells using CCT8 antibody (12263-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IF Staining of MCF-7 using 12263-1-AP

    IF Staining of MCF-7 using 12263-1-AP

    Immunofluorescent analysis of MCF-7 cells, using CCT8 antibody 12263-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inA431 cells, mouse kidney tissue, HEK-293 cells, COLO 320 cells, HeLa cells, Jurkat cells, Raji cells
Positive IP detected inJurkat cells
Positive IF detected inSW480 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12263-1-AP targets CCT8 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCT8 fusion protein Ag2926 相同性解析による交差性が予測される生物種
Full Name chaperonin containing TCP1, subunit 8 (theta)
Calculated molecular weight 548 aa, 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC012584
Gene symbol CCT8
Gene ID (NCBI) 10694
RRIDAB_906331
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCT8, also named as C21orf112, CCTQ, KIAA0002, CCT-theta, TCP-1-theta and NY-REN-15, belongs to the TCP-1 chaperonin family. It is a molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, CCT8 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.(PMID:20080638)

Protocols

Product Specific Protocols
WB protocol for CCT8 antibody 12263-1-APDownload protocol
IF protocol for CCT8 antibody 12263-1-APDownload protocol
IP protocol for CCT8 antibody 12263-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Mechanism of orphan subunit recognition during assembly quality control

Authors - Yuichi Yagita
View Article
mouseWB

Nat Genet

DYX1C1 is required for axonemal dynein assembly and ciliary motility.

Authors - Tarkar Aarti A
View Article
mouseWB

Neuron

Network organization of the huntingtin proteomic interactome in mammalian brain.

Authors - Dyna I Shirasaki
View Article
humanWB

Nat Commun

Somatic increase of CCT8 mimics proteostasis of human pluripotent stem cells and extends C. elegans lifespan.

Authors - Alireza Noormohammadi
View Article
humanWB,IF

Proc Natl Acad Sci U S A

BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.

Authors - Seo Seongjin S
View Article
humanWB

PLoS One

TDP-43 identified from a genome wide RNAi screen for SOD1 regulators.

Authors - Somalinga Balajee R BR
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Joleen (Verified Customer) (06-03-2019)

CCT8 band shows up prominently with no background.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
CCT8 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:12263-1-AP)