Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, L02 cells, human testis tissue, mouse colon tissue, Jurkat cells, human testis tissues |
| Positive IP detected in | L02 cells |
| Positive IHC detected in | human colon tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 15 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| IP | See 3 publications below |
| CoIP | See 2 publications below |
Product Information
20861-1-AP targets CCDC93 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14855 Product name: Recombinant human CCDC93 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 282-631 aa of BC028609 Sequence: CSAEIKQIVSEYAEKQSELSAEESPEKLGTSQLHRRKVISLNKQIAQKTKHLEELRASHTSLQARYNEAKKTLTELKTYSEKLDKEQAALEKIESKADPSILQNLRALVAMNENLKSQEQEFKAHCREEMTRLQQEIENLKAERAPRGDEKTLSSGEPPGTLTSAMTHDEDLDRRYNMEKEKLYKIRLLQARRNREIAILHRKIDEVPSRAELIQYQKRFIELYRQISAVHKETKQFFTLYNTLDDKKVYLEKEISLLNSIHENFSQAMASPAARDQFLRQMEQIVEGIKQSRMKMEKKKQENKMRRDQLNDQYLELLEKQRLYFKTVKEFKEEGRKNEMLLSKVKAKAS 相同性解析による交差性が予測される生物種 |
| Full Name | coiled-coil domain containing 93 |
| Calculated molecular weight | 631 aa, 73 kDa |
| Observed molecular weight | 73 kDa |
| GenBank accession number | BC028609 |
| Gene Symbol | CCDC93 |
| Gene ID (NCBI) | 54520 |
| RRID | AB_10696446 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q567U6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CCDC93 antibody 20861-1-AP | Download protocol |
| IP protocol for CCDC93 antibody 20861-1-AP | Download protocol |
| WB protocol for CCDC93 antibody 20861-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling. | ||
Nat Commun CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL. | ||
J Cell Biol Endosomal sorting of Notch receptors through COMMD9-dependent pathways modulates Notch signaling. | ||
Elife Lipid kinases VPS34 and PIKfyve coordinate a phosphoinositide cascade to regulate retriever-mediated recycling on endosomes. | ||
Mol Metab Genome-wide CRISPR screen identified a role for commander complex mediated ITGB1 recycling in basal insulin secretion. | ||
Mol Cancer Res COMMD3-mediated endosomal trafficking of HER2 inhibits the progression of ovarian carcinoma |
