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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCDC45 Monoclonal antibody

CCDC45 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A12A7

Cat no : 67755-1-Ig

Synonyms

CCDC45, DKFZp667E1824

Validation Data Gallery

Applications:
  • WB analysis using 67755-1-Ig

    WB analysis using 67755-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67755-1-Ig (CCDC45 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 67755-1-Ig

    IF Staining of U2OS using 67755-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCDC45 antibody (67755-1-Ig, Clone: 1A12A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inMOLT-4 cells, Jurkat cells, K-562 cells, HL-60 cells
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67755-1-Ig targets CCDC45 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CCDC45 fusion protein Ag27836 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 45
Calculated molecular weight 821 aa, 95 kDa
Observed molecular weight95-100 kDa
GenBank accession numberBC009518
Gene symbol CCDC45
Gene ID (NCBI) 90799
RRIDAB_2918523
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CCDC45 antibody 67755-1-IgDownload protocol
IF protocol for CCDC45 antibody 67755-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols