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- KD/KO Validated
CCDC120 Polyclonal antibody
CCDC120 Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 22041-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | Jurkat cells, mouse liver tissue |
| Positive IHC detected in | human colon tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
The immunogen of 22041-1-AP is CCDC120 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CCDC120 fusion protein Ag14319 相同性解析による交差性が予測される生物種 |
| Full Name | coiled-coil domain containing 120 |
| Calculated molecular weight | 630 aa, 68 kDa |
| Observed molecular weight | 67-70 kDa |
| GenBank accession number | BC008769 |
| Gene symbol | CCDC120 |
| Gene ID (NCBI) | 90060 |
| RRID | AB_11182175 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CCDC120, also named as JM11, is required for neurite growth. In differentiating neuroblastoma cells, CCDC120 colocalizes with CYTH2 in both neurite shaft and growth cone areas. CCDC120 has some isoforms with MW 67-70 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CCDC120 antibody 22041-1-AP | Download protocol |
| IHC protocol for CCDC120 antibody 22041-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Hierarchical assembly of centriole subdistal appendages via centrosome binding proteins CCDC120 and CCDC68. | ||
J Biol Chem Arf6 guanine-nucleotide exchange factor cytohesin-2 binds to CCDC120 and is transported along neurites to mediate neurite growth.
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