Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse kidney tissue, HeLa cells, L02 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 10 publications below |
| IHC | See 1 publications below |
Product Information
12156-1-AP targets CCBL1/KAT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2828 Product name: Recombinant human CCBL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-112 aa of BC013069 Sequence: MRHHTRPIFLFLVETGFSHVGQAGLELLTSGDPPVSASQSAGIIGVSHRTWPCSSDFDAALGAGLELTLLPRLVVGTSLSPPEPRPAGPRGRVETCPSSSPGLGGHPCLHLL 相同性解析による交差性が予測される生物種 |
| Full Name | cysteine conjugate-beta lyase, cytoplasmic |
| Calculated molecular weight | 48 kDa |
| Observed molecular weight | 48-50 kDa |
| GenBank accession number | BC013069 |
| Gene Symbol | CCBL1 |
| Gene ID (NCBI) | 883 |
| RRID | AB_10666849 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16773 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CCBL1 encodes kynurenine aminotransferase 1 (KAT1), a key enzyme involved in kynurenine pathway. CCBL1 catalyzes the production of kynurenic acid, a powerful endogenous excitatory amino acid receptor antagonist that is widely regarded as the potent neuroprotective agent.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CCBL1/KAT1 antibody 12156-1-AP | Download protocol |
| WB protocol for CCBL1/KAT1 antibody 12156-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Skeletal muscle PGC-1α1 modulates kynurenine metabolism and mediates resilience to stress-induced depression. | ||
J Intern Med Evidence that a deviation in the kynurenine pathway aggravates atherosclerotic disease in humans. | ||
Brain Behav Immun Kynurenine pathway is altered in BDNF Val66Met knock-in mice: Effect of physical exercise. | ||
Am J Physiol Cell Physiol Endurance exercise increases skeletal muscle kynurenine aminotransferases and plasma kynurenic acid in humans. | ||
Sci Rep Caffeine protects against stress-induced murine depression through activation of PPARγC1α-mediated restoration of the kynurenine pathway in the skeletal muscle. |
