Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, human placenta tissue, mouse kidney tissue, SH-SY5Y cells |
| Positive IP detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 6 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
Product Information
20774-1-AP targets CC2D1B in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14767 Product name: Recombinant human CC2D1B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 468-767 aa of BC007912 Sequence: VAATLAAAEKLASAEDSAPADKDEDEGEPPAQAPVAKKPARPTVPSSQRLPEPRASSSKESPSPSVREQLALLEARKLQYQRAALQAKRSQDLEQAKAYLRVAKWLEAQIIQARSGRPVDLSKVPSPLTDEEGDFILIHHEDLRLSQKAEEVYAQLQKMLLEQQEKCLLFSKQFMHQGNVAETTRFEKLAQDRKKQLEILQLAQAQGLDPPTHHFELKTFQTVRIFSELNSTEMHLIIVRGMNLPAPPGVTPDDLDAFVRFEFHYPNSDQAQKSKTAVVKNTNSPEFDQLFKLNINRNHR 相同性解析による交差性が予測される生物種 |
| Full Name | coiled-coil and C2 domain containing 1B |
| Calculated molecular weight | 858 aa, 94 kDa |
| Observed molecular weight | 120 kDa |
| GenBank accession number | BC007912 |
| Gene Symbol | CC2D1B |
| Gene ID (NCBI) | 200014 |
| RRID | AB_10693627 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5T0F9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CC2D1B/Freud2 and CC2D1A/Freud1 are two homologs belonging to the evolutionary conserved Lgd protein family that is involved in endosomal trafficking. CC2D1B has been identified as the novel repressor of postsynaptic 5-HT1A expression. The predicted molecular weight of CC2D1B is 89 kDa, but the observed molecular weight is around 120 kDa, which may be caused by post-translational modification. This antibody is specific to CC2D1B, which has been confirmed by siRNA test. (19423080 , 2672064)
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for CC2D1B antibody 20774-1-AP | Download protocol |
| WB protocol for CC2D1B antibody 20774-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Replication competent HIV-guided CRISPR screen identifies antiviral factors including targets of the accessory protein Nef | ||
Mol Cell Proteomics Interactome Analysis of the CC2D1A Scaffold Reveals Novel Neuronal Interactions and a Postsynaptic Role. | ||
Front Mol Neurosci Cc2d1b Contributes to the Regulation of Developmental Myelination in the Central Nervous System.
| ||
PLoS Genet The Mammalian Orthologs of Drosophila Lgd, CC2D1A and CC2D1B, Function in the Endocytic Pathway, but Their Individual Loss of Function Does Not Affect Notch Signalling.
| ||
Biol Pharm Bull Icariin regulates EMT and stem cell-like character in breast cancer through modulating lncRNA NEAT1/TGFβ/SMAD2 signaling pathway |