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Species Reactivity

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  • Featured Product
  • KD/KO Validated

CC2D1A Polyclonal antibody

CC2D1A Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16816-1-AP

Synonyms

CC2D1A, FREUD 1, Freud 1/Aki1, MRT3

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 16816-1-AP

    WB analysis of HEK-293 using 16816-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16816-1-AP (CC2D1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 16816-1-AP

    WB analysis of HeLa using 16816-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16816-1-AP (CC2D1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 16816-1-AP

    WB analysis of Jurkat using 16816-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16816-1-AP (CC2D1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 16816-1-AP

    WB analysis of HeLa using 16816-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16816-1-AP (CC2D1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 16816-1-AP

    WB analysis of mouse liver using 16816-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16816-1-AP (CC2D1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 16816-1-AP

    IP experiment of mouse liver using 16816-1-AP

    IP result of anti-CC2D1A (IP:16816-1-AP, 4ug; Detection:16816-1-AP 1:2000) with mouse liver tissue lysate 4000ug.

  • IHC staining of human testis using 16816-1-AP

    IHC staining of human testis using 16816-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with CC2D1A antibody included
  • IHC staining of human testis using 16816-1-AP

    IHC staining of human testis using 16816-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with CC2D1A antibody included
  • IHC staining of human skin using 16816-1-AP

    IHC staining of human skin using 16816-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with CC2D1A antibody included
  • IHC staining of human skin using 16816-1-AP

    IHC staining of human skin using 16816-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with CC2D1A antibody included
  • IHC staining of human brain using 16816-1-AP

    IHC staining of human brain using 16816-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with CC2D1A antibody included
  • IHC staining of human brain using 16816-1-AP

    IHC staining of human brain using 16816-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with CC2D1A antibody included
  • IHC staining of human liver using 16816-1-AP

    IHC staining of human liver using 16816-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with CC2D1A antibody included
  • IHC staining of human liver using 16816-1-AP

    IHC staining of human liver using 16816-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with CC2D1A antibody included
  • IF Staining of HepG2 using 16816-1-AP

    IF Staining of HepG2 using 16816-1-AP

    Immunofluorescent analysis of HepG2 cells, using CC2D1A antibody 16816-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman testis tissue, human brain tissue, human liver tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CC2D1A Ready-To-Use IHC Kit. CC2D1A primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16816-1-AP targets CC2D1A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CC2D1A fusion protein Ag10355 相同性解析による交差性が予測される生物種
Full Name coiled-coil and C2 domain containing 1A
Calculated molecular weight 951 aa, 104 kDa
Observed molecular weight 130 kDa, 104 kDa
GenBank accession numberBC064981
Gene symbol CC2D1A
Gene ID (NCBI) 54862
RRIDAB_2275394
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CC2D1A (coiled-coil and C2 domain-containing 1A), also known as Freud-1, Aki1 or TAPE (TBK1-associated protein in endolysosomes), is a evolutionary conserved protein located in different subcellular compartments, including the nucleus, centrosome, and endolysosomes. It acts as a scaffold protein that interacts with various proteins and plays diverse biological roles. Mutations in CC2D1A have been linked to nonsyndromic mental retardation (NMSR) and generate a truncated 85-kDa product. Several isoforms of CC2D1A proteins have also been described: 120 /130-kDa doublet of long isoform and 67 kDa short isoform. The short isoform of CC2D1A has been identified as the predominant isoform in rodent cells, while the long isoform is more abundant in human cells. Recently it has been reported that CC2D1A/TAPE is the first innate immune regulator implicated in both TLR and RLR signaling at a very early step.

Protocols

Product Specific Protocols
WB protocol for CC2D1A antibody 16816-1-APDownload protocol
IHC protocol for CC2D1A antibody 16816-1-APDownload protocol
IF protocol for CC2D1A antibody 16816-1-APDownload protocol
IP protocol for CC2D1A antibody 16816-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Anesthesiology

Astroglial MicroRNA-219-5p in the Ventral Tegmental Area Regulates Nociception in Rats.

Authors - Song Zhang
View Article
mouseWB

J Biol Chem

TBK1-associated protein in endolysosomes (TAPE)/CC2D1A is a key regulator linking RIG-I-like receptors to antiviral immunity.

Authors - Chen Kuan-Ru KR
  • KD Validated
View Article
mouseWB

Neurotherapeutics

Loss of CC2D1A in Glutamatergic Neurons Results in Autistic-Like Features in Mice.

Authors - Cheng-Yi Yang
View Article
mouseWB

J Neurosci

Conditional Deletion of CC2D1A Reduces Hippocampal Synaptic Plasticity and Impairs Cognitive Function through Rac1 Hyperactivation.

Authors - Cheng-Yi Yang
View Article