Caspase 2/p32/p18 Polyclonal antibody

Name: Caspase 2/p32/p18 Polyclonal antibody
Brand: Proteintech
SKU: 10436-1-AP

Caspase 2/p32/p18 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10436-1-AP

Synonyms

CASP 2, CASP2, Caspase 2, Caspase 2/p18, Caspase 2/p32/p18, Caspase2, ICH 1L, ICH 1L/1S, ICH1, NEDD 2, NEDD2, Protease ICH 1

Validation Data Gallery

Applications:

AllWBIPIHC

WB analysis of Jurkat using 10436-1-AP
Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 10436-1-AP (Caspase 2/p18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 10436-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 10436-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10436-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10436-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10436-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 10436-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10436-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10436-1-AP
IP result of anti-Caspase 2/p32/p18 (IP:10436-1-AP, 4ug; Detection:10436-1-AP 1:2000) with HeLa cells lysate 3000 ug.
IHC staining of human kidney using 10436-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10436-1-AP (Caspase 2/p18 antibody at dilution of 1:200 (under 10x lens).
IHC staining of human kidney using 10436-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10436-1-AP (Caspase 2/p18 antibody at dilution of 1:200 (under 40x lens).

-/ -

Cat No. 10436-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Staurosporine treated Jurkat cells, HEK-293 cells, HeLa cells, HL-60 cells, Jurkat cells, mouse kidney tissue, mouse liver tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Published Applications

WB	See 3 publications below
IF	See 1 publications below
ELISA	See 1 publications below
RIP	See 1 publications below

Product Information

10436-1-AP targets Caspase 2/p32/p18 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rabbit
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Caspase 2/p32/p18 fusion protein Ag0694 相同性解析による交差性が予測される生物種
Full Name	caspase 2, apoptosis-related cysteine peptidase
Calculated molecular weight	452 aa, 51 kDa
Observed molecular weight	48-51 kDa, 32 kDa, 18kDa
GenBank accession number	BC002427
Gene symbol	CASP2
Gene ID (NCBI)	835
RRID	AB_2228450
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CASP2(Caspase-2) is also named as ICH1, NEDD2 and belongs to the peptidase C14A family.It is involved in the activation cascade of caspases responsible for apoptosis execution and might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.It has 2 isoforms produced by alternative splicing and can exists almost as a dimer in solution(PMID:15865942).

Protocols

Product Specific Protocols
WB protocol for Caspase 2/p32/p18 antibody 10436-1-AP	Download protocol
IHC protocol for Caspase 2/p32/p18 antibody 10436-1-AP	Download protocol
IP protocol for Caspase 2/p32/p18 antibody 10436-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB, IF	Front Microbiol Enterovirus 71 Induces INF2 Cleavage via Activated Caspase-2 in Infected RD Cells. Authors - Bei Wang View Article
		Front Pharmacol PMID: 32210806 Authors - Wen Su View Article
rabbit	RIP	Life Sci Timolol induces necroptosis, apoptosis and senescence concentration-dependently in rabbit Limbal stem cells in vitro. Authors - Guo-Jian Jiang View Article
human	ELISA	Toxicol In Vitro PMID: 32320760 Authors - Ting Yang View Article
mouse	WB	Toxicon Involvement of caspase in patulin-induced hepatotoxicity in vitro and in vivo. Authors - Baigang Zhang View Article
mouse	WB	Brain Res miR-1247-3p mediates apoptosis of cerebral neurons by targeting caspase-2 in stroke. Authors - Rong Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Caspase 2/p32/p18 Polyclonal antibody

Caspase 2/p32/p18 Polyclonal Antibody for IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis of Jurkat using 10436-1-AP

WB analysis of HL-60 using 10436-1-AP

WB analysis of mouse liver using 10436-1-AP

WB analysis of HEK-293 using 10436-1-AP

WB analysis of Jurkat using 10436-1-AP

WB analysis of HeLa using 10436-1-AP

WB analysis of mouse kidney using 10436-1-AP

WB analysis of HEK-293 using 10436-1-AP

IP experiment of HeLa using 10436-1-AP

IHC staining of human kidney using 10436-1-AP

IHC staining of human kidney using 10436-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Enterovirus 71 Induces INF2 Cleavage via Activated Caspase-2 in Infected RD Cells.

PMID: 32210806

Timolol induces necroptosis, apoptosis and senescence concentration-dependently in rabbit Limbal stem cells in vitro.

PMID: 32320760

Involvement of caspase in patulin-induced hepatotoxicity in vitro and in vivo.

miR-1247-3p mediates apoptosis of cerebral neurons by targeting caspase-2 in stroke.