• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

CARS Polyclonal antibody

CARS Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15296-1-AP

Synonyms

CARS, CARS1, CYSRS, Cysteine tRNA ligase, cysteinyl tRNA synthetase

Validation Data Gallery

Applications:
  • WB analysis using 15296-1-AP

    WB analysis using 15296-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15296-1-AP (CARS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 15296-1-AP

    IHC staining of mouse brain using 15296-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15296-1-AP (CARS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 15296-1-AP

    IHC staining of mouse brain using 15296-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15296-1-AP (CARS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 15296-1-AP

    IHC staining of human pancreas cancer using 15296-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15296-1-AP (CARS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 15296-1-AP

    IHC staining of human pancreas cancer using 15296-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15296-1-AP (CARS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 15296-1-AP

    IF Staining of MCF-7 using 15296-1-AP

    Immunofluorescent analysis of MCF-7 cells, using CARS antibody 15296-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inA549 cells, HeLa cells
Positive IHC detected inmouse brain tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15296-1-AP targets CARS in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CARS fusion protein Ag7546 相同性解析による交差性が予測される生物種
Full Name cysteinyl-tRNA synthetase
Calculated molecular weight 89 kDa
Observed molecular weight 70-85 kDa
GenBank accession numberBC002880
Gene symbol CARS
Gene ID (NCBI) 833
RRIDAB_2068455
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CARS antibody 15296-1-APDownload protocol
IHC protocol for CARS antibody 15296-1-APDownload protocol
IF protocol for CARS antibody 15296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

CARS senses cysteine deprivation to activate AMPK for cell survival.

Authors - Mengqiu Yuan
  • KD Validated
View Article
humanWB,IP

Ann Rheum Dis

Mass spectrometry-based identification of new anti-Ly and known antisynthetase autoantibodies

Authors - Jean-Baptiste Vulsteke
View Article