Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | mouse brain tissue, HepG2 cells, MCF-7 cells, mouse heart tissue, mouse liver tissue, mouse ovary tissue, mouse skeletal muscle tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
Product Information
15948-1-AP targets CAPZA2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CAPZA2 fusion protein Ag8544 相同性解析による交差性が予測される生物種 |
| Full Name | capping protein (actin filament) muscle Z-line, alpha 2 |
| Calculated molecular weight | 286 aa, 33 kDa |
| Observed molecular weight | 33-40 kDa |
| GenBank accession number | BC005338 |
| Gene Symbol | CAPZA2 |
| Gene ID (NCBI) | 830 |
| RRID | AB_2070148 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P47755 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CAPZA2 antibody 15948-1-AP | Download protocol |
| IHC protocol for CAPZA2 antibody 15948-1-AP | Download protocol |
| IP protocol for CAPZA2 antibody 15948-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncotarget Genes co-amplified with ERBB2 or MET as novel potential cancer-promoting genes in gastric cancer.
| ||
Biochem J Cytoskeleton regulators CAPZA2 and INF2 associate with CFTR to control its plasma membrane levels under EPAC1 activation. | ||