Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of HeLa using 10538-1-AP
WB result of Calpain 1 antibody (10538-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calpain 1 transfected HeLa cells.
WB analysis using 10538-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 10538-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 10538-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A2780 using 10538-1-AP
A2780 cells were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10538-1-AP
IP result of anti-Calpain 1 (IP:10538-1-AP, 4ug; Detection:10538-1-AP 1:1000) with HeLa cells lysate 1200ug.
IHC staining of human urothelial carcinoma using 10538-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10538-1-AP (Calpain 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 10538-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10538-1-AP (Calpain 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 10538-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 10538-1-AP (Calpain 1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 10538-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 10538-1-AP (Calpain 1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 10538-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10538-1-AP (Calpain 1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 10538-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10538-1-AP (Calpain 1 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 10538-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Calpain 1 antibody (10538-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
FC experiment of HeLa using 10538-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.4 ug Calpain 1 Polyclonal antibody (10538-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

Calpain 1 Polyclonal antibody

Calpain 1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10538-1-AP

Publications(43)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

CAPN1, Calcium-activated neutral proteinase 1, Calpain 1 catalytic subunit, Calpain 1 large subunit, Calpain mu type

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 647

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (43)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, A2780 cells, A431 cells, mouse testis tissue, HepG2 cells, PC-3 cells, K-562 cells, Jurkat cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human urothelial carcinoma tissue, human heart tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 40 publications below
IHC	See 4 publications below
IF	See 9 publications below
CoIP	See 1 publications below

Product Information

10538-1-AP targets Calpain 1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, rabbit, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0810 Product name: Recombinant human CAPN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 413-714 aa of BC008751 Sequence: RESGCSFVLALMQKHRRRERRFGRDMETIGFAVYEVPPELVGQPAVHLKRDFFLANASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKEGDFVLRFFSEKSAGTVELDDQIQANLPDEQVLSEEEIDENFNALFRQLAGEDMEISVKELRTILNRIISKHKDLRTKGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLSIFRKFDLDKSGSMSAYEMRMAIESAGFKLNKKLYELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKTLDTDLDGVVTFDLFKWLQLTMFA 相同性解析による交差性が予測される生物種
Full Name	calpain 1, (mu/I) large subunit
Calculated molecular weight	704 aa, 82 kDa
Observed molecular weight	75 kDa
GenBank accession number	BC008751
Gene Symbol	CAPN1
Gene ID (NCBI)	823
RRID	AB_10858627
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P07384
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for Calpain 1 antibody 10538-1-AP	Download protocol
IHC protocol for Calpain 1 antibody 10538-1-AP	Download protocol
IP protocol for Calpain 1 antibody 10538-1-AP	Download protocol
WB protocol for Calpain 1 antibody 10538-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse,human	WB,IHC	J Ginseng Res Ginsenoside Rg1 ameliorates chronic intermittent hypoxia-induced vascular endothelial dysfunction by suppressing the formation of mitochondrial reactive oxygen species through the calpain-1 pathway Authors - Fang Zhao View Article
mouse	WB	Pharmacol Res Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD. Authors - Juan Wang View Article
mouse	WB	Pharmacol Res TRPC1 contributes to endotoxemia-induced myocardial dysfunction via mediating myocardial apoptosis and autophagy. Authors - Wen Tian View Article
mouse	WB	Int Immunopharmacol The role of EndophilinA1 in chronic unpredicted mild stress-induced depression model mice Authors - Mengqing Liu View Article
human	WB	J Biol Chem Calpain cleaves most components in the multiple aminoacyl-tRNA synthetase complex and affects their functions. Authors - Hui-Yan Lei View Article
mouse,human	WB	Biochim Biophys Acta Mol Cell Res Elastic titin properties and protein quality control in the aging heart. Authors - Senem Salcan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 10538-1-AP

WB analysis using 10538-1-AP

WB analysis of A431 using 10538-1-AP

WB analysis of mouse testis using 10538-1-AP

WB analysis of A2780 using 10538-1-AP

IP experiment of HeLa using 10538-1-AP

IHC staining of human urothelial carcinoma using 10538-1-AP

IHC staining of human urothelial carcinoma using 10538-1-AP

IHC staining of human heart using 10538-1-AP

IHC staining of human heart using 10538-1-AP

IHC staining of human testis using 10538-1-AP

IHC staining of human testis using 10538-1-AP

IF Staining of HeLa using 10538-1-AP

FC experiment of HeLa using 10538-1-AP

Calpain 1 Polyclonal antibody

Calpain 1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Ginsenoside Rg1 ameliorates chronic intermittent hypoxia-induced vascular endothelial dysfunction by suppressing the formation of mitochondrial reactive oxygen species through the calpain-1 pathway

Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD.

TRPC1 contributes to endotoxemia-induced myocardial dysfunction via mediating myocardial apoptosis and autophagy.

The role of EndophilinA1 in chronic unpredicted mild stress-induced depression model mice

Calpain cleaves most components in the multiple aminoacyl-tRNA synthetase complex and affects their functions.

Elastic titin properties and protein quality control in the aging heart.