Validation Data Gallery

Filter: AllWBIPIHCFC (Intra)

WB analysis using 10541-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10541-1-AP (Calmodulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10541-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 10541-1-AP (Calmodulin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10541-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10541-1-AP (Calmodulin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of NCCIT using 10541-1-AP
NCCIT cell were subjected to SDS PAGE followed by western blot with 10541-1-AP (Calmodulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10541-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10541-1-AP (Calmodulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10541-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10541-1-AP (Calmodulin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of Ramos using 10541-1-AP
IP result of anti-Calmodulin (IP:10541-1-AP, 2ug; Detection:10541-1-AP 1:600) with Ramos cells lysate 1120 ug.
IHC staining of human breast cancer using 10541-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10541-1-AP (Calmodulin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10541-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10541-1-AP (Calmodulin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 10541-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10541-1-AP (Calmodulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 10541-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10541-1-AP (Calmodulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of MCF-7 using 10541-1-AP
1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human Calmodulin (10541-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
FC experiment of HeLa using 10541-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Calmodulin (10541-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Calmodulin Polyclonal antibody

Calmodulin Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Cat No. 10541-1-AP

Publications(18)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, FC (Intra), IP, CoIP, ELISA

Synonyms

CALM3, CALML2, Calmodulin 1/2/3, Calmodulin 3, CaM

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (18)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse skeletal muscle tissue, HepG2 cells, mouse brain tissue, MCF-7 cells, HeLa cells, NCCIT cells, rat skeletal muscle tissue
Positive IP detected in	Ramos cells
Positive IHC detected in	human breast cancer tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	HeLa cells, MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 11 publications below
IHC	See 4 publications below
IP	See 2 publications below
CoIP	See 1 publications below

Product Information

10541-1-AP targets Calmodulin in WB, IHC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0827 Product name: Recombinant human CALM3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-147 aa of BC006182 Sequence: MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDESLSMPLISSFCPRLFHPCLPRPDAFIS 相同性解析による交差性が予測される生物種
Full Name	calmodulin 3 (phosphorylase kinase, delta)
Calculated molecular weight	17 kDa
Observed molecular weight	17 kDa
GenBank accession number	BC006182
Gene Symbol	Calmodulin 3
Gene ID (NCBI)	808
RRID	AB_2069442
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P62158
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Calmodulin (CaM) is a Ca(2+)-binding protein that transduces Ca2+-mediated signals by binding to and regulating the activity of hundreds of enzymes and non-enzymatic proteins. It is highly conserved across species and involved in many biological processes, including vesicle release, cell proliferation, and apoptosis. This antibody can recognize CALM1, CALM2 and CALM3.

Protocols

Product Specific Protocols
IHC protocol for Calmodulin antibody 10541-1-AP	Download protocol
IP protocol for Calmodulin antibody 10541-1-AP	Download protocol
WB protocol for Calmodulin antibody 10541-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis Authors - Ji Geng View Article
human	WB	Nat Commun Selectively targeting the AdipoR2-CaM-CaMKII-NOS3 axis by SCM-198 as a rapid-acting therapy for advanced acute liver failure Authors - Rui Wang View Article
human		Autophagy Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy Authors - Kui Wang View Article
human	WB,IHC	Cancer Cell Int CALM1 promotes progression and dampens chemosensitivity to EGFR inhibitor in esophageal squamous cell carcinoma. Authors - Tao Liu View Article
	IP	Neurochem Int Alpha-synuclein overexpression increases phospho-protein phosphatase 2A levels via formation of calmodulin/Src complex. Authors - W Yang View Article
human	WB	Biochem Biophys Res Commun The NMDAR GluN1-1a C-terminus binds to CaM and regulates synaptic function. Authors - Liang Zhou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10541-1-AP

WB analysis of MCF-7 using 10541-1-AP

WB analysis of HeLa using 10541-1-AP

WB analysis of NCCIT using 10541-1-AP

WB analysis of mouse brain using 10541-1-AP

WB analysis of HepG2 using 10541-1-AP

IP experiment of Ramos using 10541-1-AP

IHC staining of human breast cancer using 10541-1-AP

IHC staining of human breast cancer using 10541-1-AP

IHC staining of mouse testis using 10541-1-AP

IHC staining of mouse testis using 10541-1-AP

FC experiment of MCF-7 using 10541-1-AP

FC experiment of HeLa using 10541-1-AP

Calmodulin Polyclonal antibody

Calmodulin Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis

Selectively targeting the AdipoR2-CaM-CaMKII-NOS3 axis by SCM-198 as a rapid-acting therapy for advanced acute liver failure

Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy

CALM1 promotes progression and dampens chemosensitivity to EGFR inhibitor in esophageal squamous cell carcinoma.

Alpha-synuclein overexpression increases phospho-protein phosphatase 2A levels via formation of calmodulin/Src complex.

The NMDAR GluN1-1a C-terminus binds to CaM and regulates synaptic function.