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Carbonic Anhydrase IX/CA9 Polyclonal antibody

Carbonic Anhydrase IX/CA9 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11071-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

CA9, CAIX, carbonic anhydrase IX, CA 9, CA IX

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11071-1-AP targets Carbonic Anhydrase IX/CA9 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Carbonic Anhydrase IX/CA9 fusion protein Ag1540 相同性解析による交差性が予測される生物種
Full Name carbonic anhydrase IX
Calculated molecular weight 459 aa, 50 kDa
Observed molecular weight50 kDa, 60-70 kDa
GenBank accession numberBC014950
Gene Symbol CA9
Gene ID (NCBI) 768
RRIDAB_2066528
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16790
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CA9 (Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia (PMID:18703501). It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma. The protein is present in the cytoplasmic membrane and the nucleus, with a molecular weight of 50 kDa. The molecular weight of the glycosylated form is approximately 60-70 kDa. (PMID: 31142270, PMID: 31819036)

Protocols

Product Specific Protocols
WB protocol for Carbonic Anhydrase IX/CA9 antibody 11071-1-APDownload protocol
IHC protocol for Carbonic Anhydrase IX/CA9 antibody 11071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Proc Natl Acad Sci U S A

CRLX101 nanoparticles localize in human tumors and not in adjacent, nonneoplastic tissue after intravenous dosing.

Authors - Andrew J Clark
humanWB

Cell Commun Signal

KCNJ2/HIF1α positive-feedback loop promotes the metastasis of osteosarcoma

Authors - Mao Shen
humanWB

Biomed Pharmacother

Para-toluenesulfonamide, a novel potent carbonic anhydrase inhibitor, improves hypoxia-induced metastatic breast cancer cell viability and prevents resistance to αPD-1 therapy in triple-negative breast cancer

Authors - Hsin-Yuan Chen
mouse,humanWB,IHC,IF

Oxid Med Cell Longev

Carbonic Anhydrase IX Controls Vulnerability to Ferroptosis in Gefitinib-Resistant Lung Cancer

Authors - Chen Zhang
mouseIHC

Oxid Med Cell Longev

Targeting the Ang2/Tie2 Axis with Tanshinone IIA Elicits Vascular Normalization in Ischemic Injury and Colon Cancer.

Authors - Wei Zou
mouseIHC,IF

Eur J Nucl Med Mol Imaging

In vivo three-dimensional evaluation of tumour hypoxia in nasopharyngeal carcinomas using FMT-CT and MSOT.

Authors - Wenhui Huang
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