Validation Data Gallery

Filter: AllWBIHC

WB analysis using 24479-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24479-1-AP (GID8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 24479-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24479-1-AP (GID8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 24479-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24479-1-AP (GID8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 24479-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24479-1-AP (GID8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 24479-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24479-1-AP (GID8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GID8 Polyclonal antibody

GID8 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 24479-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

C20orf11, GID8, Protein C20orf11, TWA1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, K-562 cells, HeLa cells, Jurkat cells, mouse brain tissue, rat brain tissue, SW480 cells, HepG2 cells
Positive IHC detected in	mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

24479-1-AP targets GID8 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GID8 fusion protein Ag19751 相同性解析による交差性が予測される生物種
Full Name	chromosome 20 open reading frame 11
Calculated molecular weight	228 aa, 27 kDa
Observed molecular weight	30 kDa
GenBank accession number	BC032120
Gene Symbol	GID8
Gene ID (NCBI)	54994
RRID	AB_2879562
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NWU2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for GID8 antibody 24479-1-AP	Download protocol
IHC protocol for GID8 antibody 24479-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	PLoS Genet Role of Bicaudal C1 in renal gluconeogenesis and its novel interaction with the CTLH complex. Authors - Lucia Carolina Leal-Esteban View Article
human	WB	Sci Rep Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin. Authors - Shimaa H A Soliman View Article
human	WB	bioRxiv Genome-wide screen of Mycobacterium tuberculosis- infected macrophages identified the GID/CTLH complex as a determinant of intracellular bacterial growth Authors - Nelson V Simwela View Article
human	WB,IHC,IF	Cancer Lett YTHDF1 regulates GID8-mediated glutamine metabolism to promote colorectal cancer progression in a m6A-dependent manner Authors - Yicun Han View Article
human	WB	Mol Cell mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway Authors - Sang Ah Yi View Article
mouse	WB	Nat Commun Genome-wide screen of Mycobacterium tuberculosis-infected macrophages revealed GID/CTLH complex-mediated modulation of bacterial growth Authors - Nelson V Simwela View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 24479-1-AP

WB analysis using 24479-1-AP

WB analysis using 24479-1-AP

IHC staining of mouse testis using 24479-1-AP

IHC staining of mouse testis using 24479-1-AP

GID8 Polyclonal antibody

GID8 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Role of Bicaudal C1 in renal gluconeogenesis and its novel interaction with the CTLH complex.

Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin.

Genome-wide screen of Mycobacterium tuberculosis- infected macrophages identified the GID/CTLH complex as a determinant of intracellular bacterial growth

YTHDF1 regulates GID8-mediated glutamine metabolism to promote colorectal cancer progression in a m6A-dependent manner

mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway

Genome-wide screen of Mycobacterium tuberculosis-infected macrophages revealed GID/CTLH complex-mediated modulation of bacterial growth