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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C1orf58 Polyclonal antibody

C1orf58 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26109-1-AP

Synonyms

BROFTI, Brox, C1orf58, RP11 452F19.1

Validation Data Gallery

Applications:
  • WB analysis using 26109-1-AP

    WB analysis using 26109-1-AP

    HEK293 cell lysates were subjected to SDS PAGE followed by western blot with 26109-1-AP (C1orf58 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 26109-1-AP

    WB analysis of mouse brain using 26109-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 26109-1-AP (C1orf58 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 26109-1-AP

    IP experiment of HepG2 using 26109-1-AP

    IP result of anti-C1orf58 (IP:26109-1-AP, 4ug; Detection:26109-1-AP 1:300) with HepG2 cells lysate 1680 ug.

  • IHC staining of human breast cancer using 26109-1-AP

    IHC staining of human breast cancer using 26109-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26109-1-AP (C1orf58 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 26109-1-AP

    IHC staining of human breast cancer using 26109-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26109-1-AP (C1orf58 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 26109-1-AP

    IF Staining of A431 using 26109-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using 26109-1-AP (C1orf58 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26109-1-AP is C1orf58 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C1orf58 fusion protein Ag23443 相同性解析による交差性が予測される生物種
Full Name chromosome 1 open reading frame 58
Observed molecular weight 46 kDa
GenBank accession numberBC113635
Gene symbol C1orf58
Gene ID (NCBI) 148362
RRIDAB_2880382
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C1orf58 antibody 26109-1-APDownload protocol
IHC protocol for C1orf58 antibody 26109-1-APDownload protocol
IF protocol for C1orf58 antibody 26109-1-APDownload protocol
IP protocol for C1orf58 antibody 26109-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols