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  • KD/KO Validated

COA6 Polyclonal antibody

COA6 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24209-1-AP

Synonyms

C1orf31, COA6, Cytochrome c oxidase assembly factor 6 homolog



Tested Applications

Positive WB detected inHEK-293 cells, mouse liver tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

24209-1-AP targets COA6 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COA6 fusion protein Ag21525 相同性解析による交差性が予測される生物種
Full Name chromosome 1 open reading frame 31
Calculated molecular weight 125 aa, 14 kDa
Observed molecular weight 10 - 18 kDa
GenBank accession numberBC116455
Gene symbol C1orf31
Gene ID (NCBI) 388753
RRIDAB_2879459
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C1orf31, also known as COA6, is a conserved mitochondrial protein required for respiratory complex IV biogenesis. Recently study reported that C1orf31 is required for maintenance of cytochrome c oxidase (CcO) subunit levels, and has role in the Cu delivery pathway to CcO. Defects in C1orf31 is associated with mitochondrial respiratory chain disease (MRCD). (24549041)

Protocols

Product Specific Protocols
WB protocol for COA6 antibody 24209-1-APDownload protocol
IHC protocol for COA6 antibody 24209-1-APDownload protocol
IF protocol for COA6 antibody 24209-1-APDownload protocol
IP protocol for COA6 antibody 24209-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Hum Mol Genet

Copper supplementation restores cytochrome c oxidase assembly defect in a mitochondrial disease model of COA6 deficiency.

Authors - Alok Ghosh
  • KD Validated
humanWB

Cell Rep

COA6 Is Structurally Tuned to Function as a Thiol-Disulfide Oxidoreductase in Copper Delivery to Mitochondrial Cytochrome c Oxidase.

Authors - Shivatheja Soma
humanWB,IF

Int J Mol Sci

High Expression of COA6 Is Related to Unfavorable Prognosis and Enhanced Oxidative Phosphorylation in Lung Adenocarcinoma

Authors - Ming Zhang
  • KD Validated