Validation Data Gallery

Filter: AllWBIF/ICC

WB analysis using 11602-1-AP
human plasma was subjected to SDS PAGE followed by western blot with 11602-1-AP (C1qA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of human plasma using 11602-1-AP
human plasma were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human spleen using 11602-1-AP
human spleen tissue were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 11602-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 11602-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human lung using 11602-1-AP
human lung tissue were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of human colon using 11602-1-AP
human colon tissue were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IF Staining of HepG2 using 11602-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using C1QA antibody (11602-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

C1QA Polyclonal antibody

C1QA Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 11602-1-AP

Publications(16)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Complement C1q A chain

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (16)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human plasma, human colon tissue, human heart tissue, human liver tissue, human lung tissue, human spleen tissue
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 14 publications below
IF	See 6 publications below

Product Information

11602-1-AP targets C1QA in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2141 Product name: Recombinant human C1QA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-245 aa of BC030153 Sequence: MEGPRGWLVLCVLAISLASMVTEDLCRAPDGKKGEAGRPGRRGRPGLKGEQGEPGAPGIRTGIQGLKGDQGEPGPSGNPGKVGYPGPSGPLGARGIPGIKGTKGSPGNIKDQPRPAFSAIRRNPPMGGNVVIFDTVITNQEEPYQNHSGRFVCTVPGYYYFTFQVLSQWEICLSIVSSSRGQVRRSLGFCDTTNKGLFQVVSGGMVLQLQQGDQVWVEKDPKKGHIYQGSEADSVFSGFLIFPSA 相同性解析による交差性が予測される生物種
Full Name	complement component 1, q subcomponent, A chain
Calculated molecular weight	245 aa, 26 kDa
Observed molecular weight	26-30 kDa
GenBank accession number	BC030153
Gene Symbol	C1QA
Gene ID (NCBI)	712
RRID	AB_2067153
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P02745
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qA which is the A-chain polypeptide of human complement subcomponent C1q.

Protocols

Product Specific Protocols
IF protocol for C1QA antibody 11602-1-AP	Download protocol
WB protocol for C1QA antibody 11602-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Brain Behav Immun PDE4 inhibition alleviates HMGB1/C1q/C3-mediated excessive phagocytic pruning of synapses by microglia and depressive-like behaviors in mice Authors - Qian Zhao View Article
	WB	Neuron TREM2-independent microgliosis promotes tau-mediated neurodegeneration in the presence of ApoE4 Authors - Maud Gratuze View Article
	WB	Cell Mol Life Sci Dietary intake of α-ketoglutarate ameliorates α-synuclein pathology in mouse models of Parkinson's disease Authors - Wenlong Zhang View Article
	WB	J Neurosci Immune quiescence of the brain is set by astroglial connexin 43. Authors - Anne-Cécile Boulay View Article
	WB	Life Sci Cognitive impairment in Alzheimer's disease FAD4T mouse model: Synaptic loss facilitated by activated microglia via C1qA Authors - Cui Zhang View Article
human	WB,IF	CNS Neurosci Ther STING Driving Synaptic Phagocytosis of Hippocampal Microglia/Macrophages Contributes to Cognitive Impairment in Sepsis-Associated Encephalopathy in Mice Authors - Xin Lv View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11602-1-AP

WB analysis of human plasma using 11602-1-AP

WB analysis of human spleen using 11602-1-AP

WB analysis of human liver using 11602-1-AP

WB analysis of human heart using 11602-1-AP

WB analysis of human lung using 11602-1-AP

WB analysis of human colon using 11602-1-AP

IF Staining of HepG2 using 11602-1-AP

C1QA Polyclonal antibody

C1QA Polyclonal Antibody for WB, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

PDE4 inhibition alleviates HMGB1/C1q/C3-mediated excessive phagocytic pruning of synapses by microglia and depressive-like behaviors in mice

TREM2-independent microgliosis promotes tau-mediated neurodegeneration in the presence of ApoE4

Dietary intake of α-ketoglutarate ameliorates α-synuclein pathology in mouse models of Parkinson's disease

Immune quiescence of the brain is set by astroglial connexin 43.

Cognitive impairment in Alzheimer's disease FAD4T mouse model: Synaptic loss facilitated by activated microglia via C1qA

STING Driving Synaptic Phagocytosis of Hippocampal Microglia/Macrophages Contributes to Cognitive Impairment in Sepsis-Associated Encephalopathy in Mice