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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Nesprin 3 Monoclonal antibody

Nesprin 3 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1D1G12

Cat no : 68420-1-Ig

Synonyms

C14orf49, FLJ25605, Nesprin 3

Validation Data Gallery

Applications:
  • WB analysis using 68420-1-Ig

    WB analysis using 68420-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68420-1-Ig (C14orf49 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse testis using 68420-1-Ig

    IF Staining of mouse testis using 68420-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Nesprin 3 antibody (68420-1-Ig, Clone: 1D1G12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, MOLT-4 cells, C2C12 cells, PC-12 cells, rat testis tissue
Positive IF detected inmouse testis tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

68420-1-Ig targets Nesprin 3 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Nesprin 3 fusion protein Ag26012 相同性解析による交差性が予測される生物種
Full Name chromosome 14 open reading frame 49
Observed molecular weight110 kDa
GenBank accession numberBC146604
Gene symbol C14orf49
Gene ID (NCBI) 161176
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nesprin 3 also known as C14orf49, SYNE3, belongs to the Nesprin family. Nesprin-3 is a type II transmembrane protein of the outer nuclear membrane and contains a C-terminal KASH domain and a series of spectrin repeats(PMID: 16330710). Nesprin-3, a protein that links intermediate filaments to the nucleus, regulates endothelial cell morphology, perinuclear cytoskeletal organization, and flow-induced cell polarization and migration. Nesprin-3 is robustly expressed in human aortic endothelial cells and localizes to the nuclear envelope(PMID: 21937718).

Protocols

Product Specific Protocols
WB protocol for Nesprin 3 antibody 68420-1-IgDownload protocol
IF protocol for Nesprin 3 antibody 68420-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols