Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of mouse kidney using 14808-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14808-1-AP (Hikeshi antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 14808-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 14808-1-AP (Hikeshi antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 14808-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14808-1-AP (Hikeshi antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 14808-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 14808-1-AP (Hikeshi antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 14808-1-AP
IP result of anti-Hikeshi (IP:14808-1-AP, 4ug; Detection:14808-1-AP 1:500) with mouse brain tissue lysate 2640ug.
IHC staining of human kidney using 14808-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 14808-1-AP (Hikeshi antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 14808-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 14808-1-AP (Hikeshi antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung using 14808-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14808-1-AP (Hikeshi antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 14808-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14808-1-AP (Hikeshi antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

Hikeshi Polyclonal antibody

Hikeshi Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 14808-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

C11orf73, HSPC138, HSPC179, HSPC248, L7RN6

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse kidney tissue, A375 cells, mouse brain tissue, PC-3 cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human lung tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 4 publications below
IHC	See 1 publications below

Product Information

14808-1-AP targets Hikeshi in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6530 Product name: Recombinant human C11orf73 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-193 aa of BC001677 Sequence: MFGCLVAGRLVQTAAQQVAEDKFVFDLPDYESINHVVVFMLGTIPFPEGMGGSVYFSYPDSNGMPVWQLLGFVTNGKPSAIFKISGLKSGEGSQHPFGAMNIVRTPSVAQIGISVELLDSMAQQTPVGNAAVSSVDSFTQFTQKMLDNFYNFASSFAVSQAQMTPSPSEMFIPANVVLKWYENFQRRLAQNPL 相同性解析による交差性が予測される生物種
Full Name	chromosome 11 open reading frame 73
Calculated molecular weight	22 kDa
Observed molecular weight	22 kDa
GenBank accession number	BC001677
Gene Symbol	Hikeshi
Gene ID (NCBI)	51501
RRID	AB_10644295
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q53FT3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for Hikeshi antibody 14808-1-AP	Download protocol
IP protocol for Hikeshi antibody 14808-1-AP	Download protocol
WB protocol for Hikeshi antibody 14808-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Oncogene HSP110 promotes colorectal cancer growth through STAT3 activation. Authors - K Berthenet View Article
human	WB	J Med Genet Leukoencephalopathy and early death associated with an Ashkenazi-Jewish founder mutation in the Hikeshi gene. Authors - Simon Edvardson View Article
human	WB	Int J Mol Med HIKESHI silencing can enhance mild hyperthermia sensitivity in human oral squamous cell carcinoma HSC‑3 cells. Authors - Yoshiaki Tabuchi KD Validated View Article
mouse,human	WB,IHC	Pharmaceutics Role of HIKESHI on Hyperthermia for Castration-Resistant Prostate Cancer and Application of a Novel Magnetic Nanoparticle with Carbon Nanohorn for Magnetic Hyperthermia Authors - Takashi Nagai KD Validated View Article
mouse	WB	Front Genet Nuclear Accumulation of HSP70 in Mouse Skeletal Muscles in Response to Heat Stress, Aging, and Unloading With or Without Reloading. Authors - Antonios Apostolopoulos View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse kidney using 14808-1-AP

WB analysis of A375 using 14808-1-AP

WB analysis of mouse brain using 14808-1-AP

WB analysis of PC-3 using 14808-1-AP

IP experiment of mouse brain using 14808-1-AP

IHC staining of human kidney using 14808-1-AP

IHC staining of human kidney using 14808-1-AP

IHC staining of human lung using 14808-1-AP

IHC staining of human lung using 14808-1-AP

Hikeshi Polyclonal antibody

Hikeshi Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

HSP110 promotes colorectal cancer growth through STAT3 activation.

Leukoencephalopathy and early death associated with an Ashkenazi-Jewish founder mutation in the Hikeshi gene.

HIKESHI silencing can enhance mild hyperthermia sensitivity in human oral squamous cell carcinoma HSC‑3 cells.

Role of HIKESHI on Hyperthermia for Castration-Resistant Prostate Cancer and Application of a Novel Magnetic Nanoparticle with Carbon Nanohorn for Magnetic Hyperthermia

Nuclear Accumulation of HSP70 in Mouse Skeletal Muscles in Response to Heat Stress, Aging, and Unloading With or Without Reloading.