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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BTF3L3-Specific Polyclonal antibody

BTF3L3-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19753-1-AP

Synonyms

BTF3L3, HUMBTFD

Validation Data Gallery

Applications:
  • WB analysis of human brain using 19753-1-AP

    WB analysis of human brain using 19753-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 19753-1-AP (BTF3L3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 19753-1-AP

    WB analysis of human brain using 19753-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 19753-1-AP (BTF3L3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 19753-1-AP

    IHC staining of human cervical cancer using 19753-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19753-1-AP (BTF3L3-Specific antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human cervical cancer using 19753-1-AP

    IHC staining of human cervical cancer using 19753-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19753-1-AP (BTF3L3-Specific antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 19753-1-AP

    IHC staining of human placenta using 19753-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 19753-1-AP (BTF3L3-Specific antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 19753-1-AP

    IHC staining of human placenta using 19753-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 19753-1-AP (BTF3L3-Specific antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman cervical cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

19753-1-AP targets BTF3L3-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name basic transcription factor 3, like 3
Calculated molecular weight 23 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberQ13892
Gene symbol BTF3L3
Gene ID (NCBI) 132556
RRIDAB_10637858
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BTF3L3 belongs to the NAC-beta family. It is a general transcription factor. It is required for the initiation of transcription. The antibody has no cross reaction to BTF3.

Protocols

Product Specific Protocols
WB protocol for BTF3L3-Specific antibody 19753-1-APDownload protocol
IHC protocol for BTF3L3-Specific antibody 19753-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols