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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated BSEP Monoclonal antibody

BSEP Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3C11D5

Cat no : CL594-67512

Synonyms

ABC16, ABCB11, Bile salt export pump, BRIC2, BSEP, PFIC 2, PFIC2, PGY4, SPGP

Validation Data Gallery

Applications:
  • IF Staining of mouse liver using CL594-67512

    IF Staining of mouse liver using CL594-67512

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite®594 BSEP antibody (CL594-67512, Clone: 3C11D5 ) at dilution of 1:1000.

  • FC experiment of HeLa using CL594-67512

    FC experiment of HeLa using CL594-67512

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human BSEP (CL594-67512, Clone:3C11D5) (red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inmouse liver tissue
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67512-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:500-1:2000
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-67512 targets BSEP in IF, FC (Intra) applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BSEP fusion protein Ag29135 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family B (MDR/TAP), member 11
Calculated molecular weight 146 kDa
Observed molecular weight 150-160 kDa
GenBank accession numberNM_003742
Gene symbol ABCB11
Gene ID (NCBI) 8647
RRIDAB_2920147
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ABCB11, also named as BSEP, belongs to the ABC transporter superfamily. ABCB11 involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. Mutation of ABCB11 will cause the progressive familial intrahepatic cholestasis type 2 (PFIC2) and the benign recurrent intrahepatic cholestasis type 2 (BRIC2). Genetic variations in ABCB11 may play a role in drug-induced cholestasis causing liver damage.

Protocols

Product Specific Protocols
IF protocol for CL594 BSEP antibody CL594-67512Download protocol
FC protocol for CL594 BSEP antibody CL594-67512Download protocol
Standard Protocols
Click here to view our Standard Protocols